General Stability | Organism |
---|---|
2-mercaptoethanol and EDTA stabilize | Bacillus amyloliquefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | CdCl2 | Bacillus amyloliquefaciens | |
Cu2+ | CuCl2 | Bacillus amyloliquefaciens | |
DFP | - |
Bacillus amyloliquefaciens | |
Hg2+ | HgCl2 | Bacillus amyloliquefaciens | |
L-pyroglutamate | weak | Bacillus amyloliquefaciens | |
N-bromosuccinimide | - |
Bacillus amyloliquefaciens | |
Ni2+ | NiSO4, weak | Bacillus amyloliquefaciens | |
PCMB | - |
Bacillus amyloliquefaciens | |
PCMB | - |
Niallia circulans | |
Sodium tetrathionate | - |
Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM, weak activation | Bacillus amyloliquefaciens | |
FeCl2 | 1 mM, weak activation | Bacillus amyloliquefaciens | |
FeCl3 | 1 mM, weak activation | Bacillus amyloliquefaciens | |
MgCl2 | 1 mM, weak activation | Bacillus amyloliquefaciens | |
MnCl2 | 1 mM, weak activation | Bacillus amyloliquefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
3 * 24000, SDS-PAGE | Bacillus amyloliquefaciens |
72000 | - |
gel filtration | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus subtilis | - |
strain PCI 219, ML-208 and strain DT-39 | - |
Bacillus subtilis | - |
strain mito, strain NSI and strain tamarii | - |
Micrococcus luteus | - |
- |
- |
Niallia circulans | - |
- |
- |
Paenibacillus polymyxa | - |
- |
- |
Pimelobacter simplex | - |
- |
- |
Priestia megaterium | - |
- |
- |
Proteus vulgaris | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus amyloliquefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus amyloliquefaciens |
Storage Stability | Organism |
---|---|
-20°C, pH 7, in presence of 5 mM EDTA and 5 mM 2-mercaptoethanol, stable for 3 months | Bacillus amyloliquefaciens |
4°C, pH 7, in presence of 5 mM EDTA and 5 mM 2-mercaptoethanol, less than 50% loss of activity | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyroglutamyl-4-methylumbelliferyl ester + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
pyroglutamyl-Ala + H2O | - |
Bacillus amyloliquefaciens | pyroglutamate + Ala | - |
? | |
pyroglutamyl-beta-naphthylamide + H2O | - |
Bacillus subtilis | pyroglutamate + beta-naphthylamine | - |
? | |
pyroglutamyl-D-Ala + H2O | - |
Bacillus amyloliquefaciens | pyroglutamate + D-Ala | - |
? | |
pyroglutamyl-D-Leu + H2O | - |
Bacillus amyloliquefaciens | pyroglutamate + D-Leu | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 24000, SDS-PAGE | Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 7, 30 min, 5 mM EDTA and 2-mercaptoethanol, stable | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | pH 5.5: about 50% of maximal activity, pH 10.0: about 65% of maximal activity | Bacillus amyloliquefaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | 30°C, stable in presence of 5 mM EDTA and 2-mercaptoethanol | Bacillus amyloliquefaciens |